Gram Negative bacteria have been known to cause infection and are responsible for fever-induction. The fever-induction (pyrogenic) effect is due to the presence of an endotoxin within their cell wall. Most Gram Negative bacilli are from human or animal faecal or GI tract origins. Persistent elimination of these harmful bacteria through treatment of various common household articles was demonstrated by treatment with the active Germ Solutions material. The following table illustrates the reduction in number of gram negative bacteria (reported as Gram Negative Cocci in colony forming units, CFU) from identical treated and untreated surfaces, substrates and articles commonly present in households. Samples were taken by sterile swab, cultured and enumerated. Colony counts were taken on the 1st and 2nd days with the highest counts being taken to calculate final counts. Results are reported in CFU/plate, CFU/area (CFU/plate X Dilution Factor) and CFU/Culture (CFU/area X sample quantity).29
| Article | Untreated Article | Treated Article | ||||
|---|---|---|---|---|---|---|
| CFU/plate | CFU/culture | CFU/sq in | CFU/plate | CFU/culture | CFU/sq in | |
| Sink | 528 | 5280 | 1760 | 0 | 0 | 0 |
| Toilet | 53 | 530 | 177 | 0 | 0 | 0 |
| Ceramic Countertop | 548 | 5480 | 1830 | 0 | 0 | 0 |
| Shower Curtain | 432 | 4320 | 1440 | 0 | 0 | 0 |
| Stove | 393 | 3930 | 1310 | 0 | 0 | 0 |
| Wood Cabinet | 432 | 4320 | 1440 | 0 | 0 | 0 |
| Door Knob | 393 | 3930 | 1310 | 0 | 0 | 0 |
| Stair Rail | 25 | 250 | 83 | 0 | 0 | 0 |
To determine efficacy of treatment on different surfaces, hard nonporous substrates, granite, garnet, sand, zeolites (silicate mineral), gravel and marble were spray treated with our antimicrobial chemical and dried. Chemical testing confirmed all substrates to have a substantial, uniform coating of our chemical’s active material. As all substrates are siliceous materials, zeolites were chosen to be utilized in the studies. Testing was conducted on 3 separate test stations with three challenges per station to determine average microbial reductions.